Placebo Interference

[sank_yb81]

Hi Guys,

I am suffered a lot from the placebo interference from my syrup sample. I could not resolve my impurity peaks from placebo peaks. I tried with different columns and mobile phase. My sample has 2 actives, methylparaben, propyl paraben, sorbitol, FDC red40, Sugar, Sodium Citrate and citric acid. Due to one of my active components nature i cannot go for more sample concentration and higher wavelength. My working wavelength is 205 nm. Anyone can help me to resolve this.

thanks in advance

[Gerhard Kratz]

Maybe you should get rid of the matrix effect by doing SPE sample preparation. Did you also try a HILIC column?

[sank_yb81]

Hi Gerhard,

Can you explain how to perform SPE. Because this is the first time i am going to perform.

HILIC column will work? I will try it today and get back to you

[sank_yb81]

HILIC Column also not good enough to resolve the peaks

[Gerhard Kratz]

I would do use a C18 SPE to clean the sample, using an Water as solvent. Which HILIC column you tried?

[sank_yb81]

Hi Gerhard,


I used waters atlandis silica HILIC column