-
- Posts: 26
- Joined: Tue Feb 09, 2010 9:10 pm
Advertisement
Units of UV abs. peak area in masslynx
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
3 posts
Page 1 of 1
Does anyone know the unit which masslynx reports for UV absorbance peak areas? With Chemstation (mAU*s) and Chromeleon (mAU*min) I am able to calculate back the amount of injected protein using the molar absorption coefficient of the protein (taking into account flow rate and path length of the flow cell). This doesn't work with MassLynx. (Neither mAU*s nor mAU*min seem to be the actual unit of the reported peak area.) Is "mAU" from MassLynx really "m absorbance units" or more like "m arbitrary units"? (Sample recovery is not the problem here.)
-
- Posts: 26
- Joined: Tue Feb 09, 2010 9:10 pm
Whoever is interested in:
Using Waters TUV detector in dual wavelength mode may lead to deviation
in IPA's for either WL compared to single WL mode. We observed ~15 % smaller IPA at 214 nm in dual wavelength mode. Values measured in single WL mode correspond to the ones expected from Beer-Lambert's law. (Recalibration of the detector doesn't change the situation.)
Using Waters TUV detector in dual wavelength mode may lead to deviation
in IPA's for either WL compared to single WL mode. We observed ~15 % smaller IPA at 214 nm in dual wavelength mode. Values measured in single WL mode correspond to the ones expected from Beer-Lambert's law. (Recalibration of the detector doesn't change the situation.)
-
- Posts: 4
- Joined: Thu Jun 30, 2011 11:12 am
Could you please give me an example of calculation on how to derive the mass of injected protein from the mAU*s peak area, molar absorption coefficient, path length and flow rate? I do have exactly this problem because there are no certified standards for my analyte so I can't use a calibration curve and I'm not quite sure I got right the calculations.Does anyone know the unit which masslynx reports for UV absorbance peak areas? With Chemstation (mAU*s) and Chromeleon (mAU*min) I am able to calculate back the amount of injected protein using the molar absorption coefficient of the protein (taking into account flow rate and path length of the flow cell). This doesn't work with MassLynx. (Neither mAU*s nor mAU*min seem to be the actual unit of the reported peak area.) Is "mAU" from MassLynx really "m absorbance units" or more like "m arbitrary units"? (Sample recovery is not the problem here.)
Thank you in advance.
3 posts
Page 1 of 1
Who is online
In total there are 32 users online :: 3 registered, 0 hidden and 29 guests (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am
Users browsing this forum: Ahrefs [Bot], Amazon [Bot], Google [Bot] and 29 guests
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am
Users browsing this forum: Ahrefs [Bot], Amazon [Bot], Google [Bot] and 29 guests
Latest Blog Posts from Separation Science
Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.
Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.
- Follow us on Twitter: @Sep_Science
- Follow us on Linkedin: Separation Science