Chromatography Forum

Looking to get some information from some of you experts out here. I am needing to set up some mobile phases from pH 2 to 11. I am thinking of using a 10 mM Ammonium Formate aqueous solution and using Formic Acid to generate two acidic mobile phases of pH 2 and 4.5 and using Ammonium Hydroxide to generated two basic mobile phases with pH 9 and 11. Utlimately I am needing about 5 mobile phases having pH's of 2, 4.5, 7, 9 and 11.

Does anyone see a problem with this from a LCMS perspective? The column I am using for this application is rated between pH 1 and 12. If anyone has any other mobile phases that would be easy to prepare and LC/MS compatible I would appreciate any ideas. If you think what I have will work I would appreciate hearing your feedback on that as well.

Thanks in advance.

for pH 11 you'll probably want to use ammonium bicarbonate

A. Carl Sanchez,

Thank you for your response. I will certainly give that a try.

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