Chromatography Forum

I want to know whether detector can be blocked with particles? or any other reasons can cause blocking the reactor? for example other solutions? or it can be blocked with solid particles only?

Sure. Any detector can get blocked with particles. What type/brand/model detector do you have?

[quote="sepscientologist"]Sure. Any detector can get blocked with particles. What type/brand/model detector do you have?[/q

actually my problem is that their is no particle in the line because before detector and after column I used a filter with 0.2 micrometer. so if there is any particle in the line this filter must be blocked and I myself believe particles less than this can not block the detector's cell!!!! Am I right?

I want to know detector can be blocked with particles? or some solutions or liquids can block the detector?

Sure. Any detector can get blocked with particles. What type/brand/model detector do you have?[/q

actually my problem is that their is no particle in the line because before detector and after column I used a filter with 0.2 micrometer. so if there is any particle in the line this filter must be blocked and I myself believe particles less than this can not block the detector's cell!!!! Am I right?

I want to know detector can be blocked with particles? or some solutions or liquids can block the detector?

but the point is there no any block in that filter but detector blocked hahah

What kind/brand/model detector is it?

this the brand

Waters 2414 Differential Refractometer

Yeah... don't really know that Waters detector. If it was a UV detector I would check the max back pressure
ratio then carefully flow a few solvents in reverse direction being careful not to esceed max back pressure.
Tapping on the tubing with your 1/4" wrench can be helpful. I would wait for someone else to check in before
doing it though. RI detectors can be kind of fragile. It's Waters so it should be well designed. I would expect there
to be a long narrow tube going into the detector to allow for good temp control. You might hunt around in there
for a way to disconnect just before the RI cell and backflow just the tubing. Again, I would wait for input from
someone with knowledge of this particular detector. Good luck!

precipitation?

sepscientologist
thank you and I am waiting. I want to know if it is possible some special solution block the detector (RI) as I have no any information about this detector and inside of it. I mean for example any solution can do swelling or even precipitation inside the detector I mean the cell of the detector. or only particles can block the detector?

Karen01
I do not think there is precipitation because we have a 0.2 micrometer filter before the detector if there is any precipitate after column can be trapped by this filter. we checked there is very good pressure after filter without any particle.

So, is your detector flow cell bolcked or not?

You ask: any solution can do swelling. Maybe, but not enough to block the detector.

You say: no precipitation because we have a 0.2 micrometer filter before the detector. There is no reason things cant precipitate after the detector, filtered or not. I could even imagine a situation where you dissolve or degrade the filter which then plugs the detector.

Just a few other things. The actual flow cell on the 2414 is quite pressure sensitive (as are all RID flow cells), a blockage post flow cell will damage that cell.

In my experience, blockages post column happen in the tubing inlets to the flow cell, not in the flow cell itself.

aa thanks, but till now i can not find the reason. anyway the joke thing is detector blocks and after some time it is ok, I mean alternatively.

what is your compound?
they can stick as well in the flow path
I advised recently on a case for that on a UPLC system with a flow cell not suite for proteins.

have you correctly balanced you RI?

what is your compound?
they can stick as well in the flow path
I advised recently on a case for that on a UPLC system with a flow cell not suite for proteins.

Why was the flow cell unsuited for proteins?

- Karen

hahah now it is working again, hahah i think the problem is in the flow path may there is some blocking in it, i mean fixed particles in the flow path which caused a narrow path to fluid and flow blocked by the rate and after some while it is open, i really surprised but it is on the guaranty and i will ask from them to fix it , let see what will happen after.

karen01
the flow cell used in the H-class acquity does not come fitted for all biotech applications
do HgH on it and it clogs completely.
it is not a SST flow cell like we are used to from the alliance