How to develop a HPLC method to determine Fosfomycin?

[woaixiekeshu]

Hi, the method with the Aminopropyl column and RID is so harmful to my column, and get the bad peak. who know how to How to develop a HPLC method to determine Fosfomycin with C18 column? thanks!

[woaixiekeshu]

Hi, is there anybody who can tell me the method? thank you!

[Gerhard Kratz]

Please google for that:
Study on high performance liquid chromatography/indirect photometric detection of fosfomycin with acridine as detection reagent]

Y Hu, S Da, Y Feng, Q Zhang

Department of Chemistry, Wuhan University, Wuhan, 430072.

or the following:
Determination of Fosfomycin Trometamol and its Related Substances in the Bulk Drug by Ion-Pair HPLC with Evaporative Light Scattering Detection

Journal of Liquid Chromatography & Related Technologies

Volume 29, Issue 1, 2006, Pages 15 - 24

Authors: Hao Liua; Hongwu Wangb; V. Bruce Sunderlandc

DOI: 10.1080/10826070500358209
or that one on the Waters library:
Content determination of fosfomycin calcium capsules by HPLC-ELSD


Library Number:

NP73838

Author(s):

Zhong HM;

Source:

CHINESE JOURNAL OF ANTIBIOTICS

Content Type:

Journal Citations

Year:

2006 Volume: 31(4) Page(s): S1-S2

Good luck

[woaixiekeshu]

Hi, thank you for Gerhard Kratz ! I employed the method from EP6.0 by using NH2-column and RID detection, but got the tailing peak when the standard was injected, just for three times, the column was useless，you know the column is so expensive. what should i do to prolong the life time of the column?

[Vlad Orlovsky]

Your compound is probably reacting with amino group of the stationary phase. You need amine on the column which will not react with your compound. Also hydrolysis of epoxy ring is possible. You can intentionally quench your compound and open the ring or do another form of derivatization.

[woaixiekeshu]

Thanks for your suggestion! but the method i employed according to EP6.0, is is not the best method for the analysis?