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- Posts: 3
- Joined: Fri Mar 11, 2011 10:04 am
A student of ours is planning to use our HPLC to analyse rumen blood and digesta samples (he'll try to quantify dicyandiamide or DCD, fed to cows to control methane production and post-excretion nitrification - mobile phase = water, flow rate = 0.8 ml/min, T = 30 degrees Celsius, guard column used). I'm wondering if I should get worried that these samples are going to wreck my column. I expect them to be like a sticky syrup, which is a big change from the water samples I've analysing so far. Would anyone be aware of any pre-treament (other than a 0.2 um filtration) required to minimise damages?
Regards,
eddy