Reversed phase HPLC method for Terpene hydrocarbon Myrcene

[bobby12]

I need an RP method for detection of terpene hydrocarbon myrcene. Will appreciate suggestion about column and mobile phase choice. I can not use normal phase in my current HPLC. TLC method is not very sensitive.

[tom jupille]

That would probably be better done by GC.

[Alex Buske]

Chromatographia
Volume 30, Numbers 5-6, 271-276, DOI: 10.1007/BF02319706

Originals
Analysis of lemon and bergamot essential oils by HPLC with microbore columns

M. Benincasa, F. Buiarelli, G. P. Cartoni und F. Coccioli

[bbkf]

correct me if i'm wrong but aren't those compounds non-polar? if they are you should be fine with C18. I'd start off with a water/acetonitrile mobile phase. Find out the pKa for each and choose a buffer if needed.

BTW...are those compounds from hops?

[bobby12]

Thanks all