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Reversed phase HPLC method for Terpene hydrocarbon Myrcene
Posted: Thu Jun 09, 2011 6:12 pm
by bobby12
I need an RP method for detection of terpene hydrocarbon myrcene. Will appreciate suggestion about column and mobile phase choice. I can not use normal phase in my current HPLC. TLC method is not very sensitive.
Re: Reversed phase HPLC method for Terpene hydrocarbon Myrce
Posted: Thu Jun 09, 2011 11:33 pm
by tom jupille
That would probably be better done by GC.
Re: Reversed phase HPLC method for Terpene hydrocarbon Myrce
Posted: Fri Jun 10, 2011 8:51 am
by Alex Buske
Chromatographia
Volume 30, Numbers 5-6, 271-276, DOI: 10.1007/BF02319706
Originals
Analysis of lemon and bergamot essential oils by HPLC with microbore columns
M. Benincasa, F. Buiarelli, G. P. Cartoni und F. Coccioli
Re: Reversed phase HPLC method for Terpene hydrocarbon Myrce
Posted: Fri Jun 10, 2011 5:04 pm
by bbkf
correct me if i'm wrong but aren't those compounds non-polar? if they are you should be fine with C18. I'd start off with a water/acetonitrile mobile phase. Find out the pKa for each and choose a buffer if needed.
BTW...are those compounds from hops?
Re: Reversed phase HPLC method for Terpene hydrocarbon Myrce
Posted: Fri Jun 10, 2011 5:11 pm
by bobby12
Thanks all