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kc811 acn
Posted: Tue May 31, 2011 2:28 pm
by vestelshirley
an aqueous sample diluted 1:5 with acetonitrile was mistakenly injected onto a kc811 column running 0.1% h3po4 at 70 centigrades. 10 uL was shot and there was a guard column on it .
What damage might have incurred?
Re: kc811 acn
Posted: Tue May 31, 2011 3:04 pm
by tom jupille
From a quick look at the Shodex site, it looks like that column is a sulfonated polystyrene-divinylbenzene in the hydrogen form (similar to the BioRad HPX87H). Those are very robust columns. You can inject 10 microliters of just about *anything*, and the worst-case scenario is that you would have to change the guard cartridge.
Re: kc811 acn
Posted: Tue May 31, 2011 3:28 pm
by vestelshirley
I agree. The guard column probable earned its keep, but the darned thing costs almost 500 and must be packed.
the column calls for a 0 to 20% ethanol addition with column kept below about 720 psi.
no informatin on pressure constraints for 0.1% phosphoric mobile phase.