Chromatography Forum

Hello All..I am now started to work on Amino Acids.Please anybody share their knowledge of work on Amino Acids Analysis like which Column they used and all..

Thanks
Jardin

Hello
See sea literature.
http://www.google.ru/#hl=ru&newwindow=1 ... 39&bih=779
After read this sea... If will unclear and unknown detailed and correct questions
=> "You come in , if this"

Hello Jardin,

You can use at least for approaches for analysis of amino acids:
1. Traditional RP column with ion-pairing reagent in the mobile phase (a lot of published and well tested methods, including ones in USP)
2. Mixed-mode column. In mixed-mode column, ion-pairing reagent is attached to the surface of silica gel. Columns retain amino acids by combination of weak reversed-phase and moderate cation-exchange mechanisms. Method is compatible with ELSD, LC/MS and prep chromatography. Here are few references from our website:
http://www.sielc.com/Compound-Amino-Acids.html
3. HILIC methods (a lot of methods published). Here is our approach with mixed-mode HILIC:
http://www.sielc.com/Application-Effect ... Acids.html
http://www.sielc.com/Application-HPLC-S ... isc-N.html
4. Derivatization of amino acids with consequent analysis on reversed-phase columns

Contact me with a list of AA you are trying to separate and please describe objectives of you experiment.

Hello Jardin

I just had worked on my Amino Acids Application. I got excellent analysis on the following Japanese Column. I recommend you 100% to use this column.
The link of this Application is
http://develosil.net/data/eappli002.htm

Column: Develosil RPAQUEOUS (C30-UG-5) 250 x 4.6 mm
Mobile phase: water
Flow rate: 1.0 mL/min
Temperature: 40 C
Detection: RI
Sapmle volume: 10 ul
Sample:
1. alanine 0.2%
2. valine 0.2%
3. isoleucine 0.2%
4. leucine 0.2%

Hello Dino
Jardin question - its severe quest ! We a don't know she's problem (free aminoacid or in peptide, sample (food, enviroment ), quality or quantity analysis , concentration), equipment (aminoacid analyser, standart HPLC (detectors?), she qualification ("yang girl after scool whithout work experience" or " yang lady after institute and have work experience as "operator"").
Therefore, to give advice other than itself "to view the sea of information" is difficult. This advice though in rough shape, but is useful. The method is not new, soon to be half a century. Options for implementing a huge number.Good information gathered in the old references - such as the CRC "Chromatography" or Fundamentals and Application of Chromatographic and Electrophoretic Methods (E Heftmann)

And about your proposed method. Its method requires very high qualifications of the operator, and high concentration samples, since the low sensitivity of RI.