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Peptide detection

http://www.chromforum.org/viewtopic.php?t=16627

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Peptide detection

Posted: Fri May 20, 2011 1:25 pm
by peptideismylife
Hi colleagues,

I am going to carry out the detection of a peptide. It has different ionization sites due to have several amino groups and hydroxyl groups. My question is...I have to add TFA in the mobil phase to aid protonation...or with ACN / H2O could be enough?

Thanks

Re: Peptide detection

Posted: Sun May 22, 2011 4:53 pm
by kerri
TFA is used as an ion pairing agent in peptide separation, but it isn't a requirement for every analysis. You could start with ACN and 0.1% formic acid and see if that works for you.
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