Chromatography Forum

I am searching for a HPLC method to analyze the following, DMDM Hydantoin and IPBC? Is there any methods available using uv detection?

Biggest question is: are you trying to assay incoming raw material (like Glydant Plus) or looking to quantitate in a finished product? Obviously, situation 1 is much simpler. For that, we've done IPBC using old-Type A RP 18 column, 10 cm x 4.6 mm 5 micron 50% H2O 50% Acetonitrile Flow = 1.00 ml/min. % Solvent B = 50 Wavelength = 235 nm IPBC will elute at about 4 minutes using these conditions. Peak shape should be even better with modern Type B RP-18 columns. For DMDM hydantoin, we used the traditional Nash/Hantzsch colorimetric reaction, although some have used HPLC with post-column reaction to do intact DMDM hydantoin.

We assayed for IPBC this way in product cartons, but finished products contain more interferences such as fragrances. We played with HPLC-MS for that, but there wan't much interest here.

Biggest question is: are you trying to assay incoming raw material (like Glydant Plus) or looking to quantitate in a finished product? Obviously, situation 1 is much simpler. For that, we've done IPBC using old-Type A RP 18 column, 10 cm x 4.6 mm 5 micron 50% H2O 50% Acetonitrile Flow = 1.00 ml/min. % Solvent B = 50 Wavelength = 235 nm IPBC will elute at about 4 minutes using these conditions. Peak shape should be even better with modern Type B RP-18 columns. For DMDM hydantoin, we used the traditional Nash/Hantzsch colorimetric reaction, although some have used HPLC with post-column reaction to do intact DMDM hydantoin.

We assayed for IPBC this way in product cartons, but finished products contain more interferences such as fragrances. We played with HPLC-MS for that, but there wan't much interest here.

Can you expand on the HPLC with post column reaction to do intact DMDM hydantoin? would you use the same method as above?

See Analytical Chemistry 1990, Vol 62, pp. 1397-1402 and Pickering ALboratories application #MA-325-A