Alternative to Glycine buffer (pH 9.0)

[solntzevorot]

Hi all,

I'm searching for glycine buffer alternative for mobile phase.
I'm using gradient method : sol.A Glycine buffer (pH 9.0), sol.B : Acetonityl:MeOH 85:15.

I'm searching appropriate buffer at pH 9.0 for UPLC system (but not Ammonium based).

Please help

[Kostas Petritis]

Does it need to be volatile? If not, how about Tris?

[solntzevorot]

Does it need to be volatile? If not, how about Tris?

No, it doesn't have to be volatile.

What about phosphate buffer, prepared from tri-basic and di basic phosphate?

[Kostas Petritis]

It should work as the pka's are 7.2 and 12.32, barely within the +/- 2 "rule".

[solntzevorot]

It should work as the pka's are 7.2 and 12.32, barely within the +/- 2 "rule".

Thanks

[Gerhard Kratz]

Another organic buffer is CAPSO, pKa 9,7.
Or 1-Methyl-piperidine
Or Borate, but it is not volatile.

[DSP007]

Hellow
Volature basic buffer
1) ammonia buffer (NH3/+H20/+ NH4CL) or NH3/+H20/+ (NH3)2CO3
This russian langvige , but text scool task understood without translation
http://forum.xumuk.ru/index.php?showtopic=72307
2) triethylamine buffer (C2H5)3N+H20 + CH3COOH

Borate buffer -good stabile buffer , but in LC-MS borate buffer must not use.

[unmgvar]

can you say why not ammonium based buffer?

[DSP007]

Nasty (Ungly) it is.
Volatile, malodorous, unstable and RP-silica gel "eats". But because of volatile buffers for UPLS-MS the ammonium is most volatile.

2 solntzevorot (Midsummer)=> by nick can be seen from the "brozer slavjans". Come on (part of) on anchem.ru

[HW Mueller]

Am I going bananas or did solntzevorot say his buffer need not be volatile?

[DSP007]

Hello HW Mueller.

Very likely when you worked with the ammonia buffer and know that "it's not sugar".

[solntzevorot]

Thanks to all for the replies.

The buffer no need to be volatile.

Whith ammonium buffers (at least with those I have in the lab) I experienced with to much system peaks in the UPLC system.

to DSP007 : thanks for the link, I freely speak Russian and Ukrainian. (Born in USSR )

[Kostas Petritis]

Whith ammonium buffers (at least with those I have in the lab) I experienced with to much system peaks in the UPLC system.

You shouldn't experience system peaks with ammonium buffers. If you saw peaks is probably either the low purity or contaminated salt. I would try again with some new high purity container.