Phenolic separation using reversed phase column in HPLC

[kellylen1412]

I am currently using Shodex normal reversed phase column RsPak DE-413L column for HPLC analysis. The compounds that I intend to analyze is PHENOL, GUAICOL (2-methoxyphenol), and CATECHOL(1,2-dihydroxybenzene).
The following conditions were used for the analysis: flowrate 1.0 mL/min; eluent 0.005 M HClO4 aqueous solution/CH3CN = 90/10; oven temperature 40oC; UV/vis detector 220 nm.
However, i find that the RsPak De-413L is not able to separate these compounds (phenol, catechol and guaiacol) and when analyzed in HPLC, it will come out together as 1 peak. Is there anyway to make this compounds eluted separately in HPLC. I would like advice on this matter.

[Jiri Urban]

Did you try HILIC? It might help

[DSP007]

Hi, Good afternoon.
Acidic phase, and too much water. Guajakol small soluble in water and sat down on the stationary phase. Try to wash column with pure methanol or methanol -wather 9:1 , and further use phase with 60-70% acetonitrile.

[unmgvar]

at what retention time do they come out as one peak?
is it at the front of the column?
or is it at a different retention time?
what is the pH of your aqueous solution?

depending on the answer to this you have several possibilities,

[praveenpaliwal]

I think U should try the column which have more silanol activity. Try normal phase column with Hexane methanol combination. I think It will work rather than another combination.

Best regards

Praveen