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Alternative to Glycine buffer (pH 9.0)
Posted: Sun May 15, 2011 12:19 pm
by solntzevorot
Hi all,
I'm searching for glycine buffer alternative for mobile phase.
I'm using gradient method : sol.A Glycine buffer (pH 9.0), sol.B : Acetonityl:MeOH 85:15.
I'm searching appropriate buffer at pH 9.0 for UPLC system (but not Ammonium based).
Please help
Re: Alternative to Glycine buffer (pH 9.0)
Posted: Sun May 15, 2011 4:48 pm
by Kostas Petritis
Does it need to be volatile? If not, how about Tris?
Re: Alternative to Glycine buffer (pH 9.0)
Posted: Mon May 16, 2011 5:22 am
by solntzevorot
Does it need to be volatile? If not, how about Tris?
No, it doesn't have to be volatile.
What about phosphate buffer, prepared from tri-basic and di basic phosphate?
Re: Alternative to Glycine buffer (pH 9.0)
Posted: Mon May 16, 2011 5:36 am
by Kostas Petritis
It should work as the pka's are 7.2 and 12.32, barely within the +/- 2 "rule".
Re: Alternative to Glycine buffer (pH 9.0)
Posted: Mon May 16, 2011 6:54 am
by solntzevorot
It should work as the pka's are 7.2 and 12.32, barely within the +/- 2 "rule".
Thanks
Re: Alternative to Glycine buffer (pH 9.0)
Posted: Mon May 16, 2011 6:58 am
by Gerhard Kratz
Another organic buffer is CAPSO, pKa 9,7.
Or 1-Methyl-piperidine
Or Borate, but it is not volatile.
Re: Alternative to Glycine buffer (pH 9.0)
Posted: Mon May 16, 2011 8:15 am
by DSP007
Hellow
Volature basic buffer
1) ammonia buffer (NH3/+H20/+ NH4CL) or NH3/+H20/+ (NH3)2CO3
This russian langvige , but text scool task understood without translation
http://forum.xumuk.ru/index.php?showtopic=72307
2) triethylamine buffer (C2H5)3N+H20 + CH3COOH
Borate buffer -good stabile buffer , but in LC-MS borate buffer must not use.
Re: Alternative to Glycine buffer (pH 9.0)
Posted: Mon May 16, 2011 6:27 pm
by unmgvar
can you say why not ammonium based buffer?
Re: Alternative to Glycine buffer (pH 9.0)
Posted: Tue May 17, 2011 8:29 am
by DSP007
Nasty (Ungly) it is.
Volatile, malodorous, unstable and RP-silica gel "eats". But because of volatile buffers for UPLS-MS the ammonium is most volatile.
2 solntzevorot (Midsummer)=> by nick can be seen from the "brozer slavjans". Come on (part of) on anchem.ru
Re: Alternative to Glycine buffer (pH 9.0)
Posted: Tue May 17, 2011 8:32 am
by HW Mueller
Am I going bananas or did solntzevorot say his buffer need not be volatile?
Re: Alternative to Glycine buffer (pH 9.0)
Posted: Tue May 17, 2011 9:37 am
by DSP007
Hello HW Mueller.
Very likely when you worked with the ammonia buffer and know that
"it's not sugar".
Re: Alternative to Glycine buffer (pH 9.0)
Posted: Thu May 19, 2011 4:58 am
by solntzevorot
Thanks to all for the replies.
The buffer no need to be volatile.
Whith ammonium buffers (at least with those I have in the lab) I experienced with to much system peaks in the UPLC system.
to DSP007 : thanks for the link, I freely speak Russian and Ukrainian. (Born in USSR
)
Re: Alternative to Glycine buffer (pH 9.0)
Posted: Thu May 19, 2011 5:39 am
by Kostas Petritis
Whith ammonium buffers (at least with those I have in the lab) I experienced with to much system peaks in the UPLC system.
You shouldn't experience system peaks with ammonium buffers. If you saw peaks is probably either the low purity or contaminated salt. I would try again with some new high purity container.