Chromatography Forum

Per the GPC - SEC startup kit for Agilent 1100, there is an adapter needed to connect straight to the active inlet valve if the instrument is equipped with a quat pump. Say for instance you are using channel A, would you essentially connect the solvent tube for channel A directly to the AIV? Is it absolutely essential to completely by bass the multi channel gradient valve when performing GPC analysis on an Agilent 1100 (mobile phase THF)? If someone using this setup could post a pic that would be great as I cant find a picture of this adapter anywhere.

Part Number is 0100-1847 I believe

You would connect the solvent line A directly to the AIV with this part. As to why you would want to bypass the Quat Valve I cannot answer unless, like you say there is an issue with THF ( what about the other fittings, tubing etc ).
If you are using an RID detector, then yes do bypass, there is an Agilent article about this on their website.

Regs

Hi!
The name of that part is: PEEK adapter 1/4-28 to 10-32
Reg: BWA

We typically use that adapter (#0100-1847) to bypass the mixing valve to obtain smoother baseline when we use RID or conductivity detectors.

It looks pretty similar to some fingertight nuts, so I think that's why we've lost or overlooked some. Your system came with one when new, ours did.

Thanks for the input. Now that I know what to look for, I will see if we have this adapter laying around in a drawer.

It's beige/tan/natural in color.

Now that the MCGV is bypassed, and the solvent line A is connected directly to the AIV, is it necessary to plug the solvent A connection to the MCGV? Will mobile phase from a previous run leak out of the MCGV when it is bypassed and a channel is left unplugged? Reason I ask is I dont have any spare PEEK fingertight plugs laying around. Also, can the solvent line that was disconnected ( in the center of the MCGV that ran to the AIV) just be left essentially dangling or is a second plug needed?

Now that the MCGV is bypassed, and the solvent line A is connected directly to the AIV, is it necessary to plug the solvent A connection to the MCGV? Will mobile phase from a previous run leak out of the MCGV when it is bypassed and a channel is left unplugged? Reason I ask is I dont have any spare PEEK fingertight plugs laying around. Also, can the solvent line that was disconnected ( in the center of the MCGV that ran to the AIV) just be left essentially dangling or is a second plug needed?

I plug both. My "kit" (OK, a sandwhich bag) consists of the Agilent adapter, a fingertight plug for the MCGV, and a cap (or a union and a plug) for the fitting that goes to the AIV.

Thanks Consumer Products Guy! I'm going to order some parts for my "kit" tonite. I think the fingertight plug is Agilent part number 0100-1259. Does this "cap" or "union and plug" you speak about have a part number?

Thanks Consumer Products Guy! I'm going to order some parts for my "kit" tonite. I think the fingertight plug is Agilent part number 0100-1259. Does this "cap" or "union and plug" you speak about have a part number?

Fingertight plugs came with the vacuum degasser units when new, plugged their openings. The union is a typical 1/16 inch swagelok style HPLC tubing "union" or dead volume "union", and the plug for it as same as an HPLC column plug, or the stainless steel plug you use when running a pump pressure check.

Hi

If you cannot find the plugs straightaway, don't worry too much the MCGV should not leak as long as you dont program a gradient in the chemstation and leave it on 'A'. If it does leak then your MCGV is faulty. Also be careful with the middle tubing ( thinner one ), the nut is plastic and very easily crossthreaded...which leads to all sorts of fun and games.

LCbob