Chromatography Forum

Hello, I'm new to the forum and need some help. I have an HP 1090 Series II W/ HP PLgel Mixed Bed Column and HP 5mn 100 x 2.1mn Column that I'm selling. I don't know much about 1090's but I have a guy interested that wants me to do some basic tests. I plan on going to the where I have it stored in the next couple of days to check it for him.
He wants me to power it on and then turn pump and detector "ON". After 1 minute, which LED's stay ON.

So my first question is can I harm the unit by doing this? My second question is which LED's should be on?

This unit was setup in an HP lab and working about 6 weeks ago. It was only removed from the lab one week ago. HP tells me it's a good working unit but about all I know for sure is how to plug it in to power (I think ).

Any help would be greatly appreciated.

Thanks
rwf

Hello. This theme is rubbed repeatedly, use the search by type of instrument.
1) Turn-connect the cable to an electrical outlet and press the on / off.
It is important, check the voltage of power! In the U.S. it is 110V AC in Europe and Russia, 230V AC. The device is likely to have meets both the switch, as produced for both markets.

2) Important! Do not give the pump to work "on a dry, piston rings abrasive erased at one time.
Before turning the electricity necessary to take three bottles, fill them with water, disconnected from the pumps feeding tube (clear plastic) and allow water to drain down (to suck the water hose spritsovkoy). After that, screw-filled tube back.
3) is important. If the device is a long time not worked or brought in from the cold - it is important to sustain it at room temperature until the moisture evaporates from the boards.

Be aware the device is very old - prepare for possible problems.

One thing I ran into when I tried to revive a 1090 was that it requires an airsource to come up without throwing errors. The valves in that thing were run pneumatically iirc and without the air to run them it would be generally unhappy and instead of the 3 orange LEDs that would light up you would get 3 red ones.

One thing I ran into when I tried to revive a 1090 was that it requires an airsource to come up without throwing errors. The valves in that thing were run pneumatically iirc and without the air to run them it would be generally unhappy and instead of the 3 orange LEDs that would light up you would get 3 red ones.

It needs air source for the sampler and Helium (for degasing the mobile phase).
There is no pistons in this pump. It is a diaphram pump high pressure with three metering pumps.
This is the best HPLC pump ever built by any menufectrer, whith no ripple to talk about.
you can run 50uL/min flow gradients very precise and accurate.
The reason thay do not make is anymore is because of the cost. The menufecturer realize that quote 'almost all chemists do not have fluide mechanics education' (stupid), and went back to pistons afraid that thay would be priced out of the market with the cheep piston high presure pump.
This is a big loss for chromatography.