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Mobile phase of HPLC
Posted: Thu Apr 28, 2011 2:53 am
by weitze
I am trying to test concentration of nitrate and nitrite by HPLC Agilent 1200. I am using column ODS Hypersil column. I had tried 70% methanol and 30% water as my mobile phase, but the retention time is different for various concentration standard solution. Does this means that I used wrong mobile phase?
Can anybody suggest me what the mobile phase that I should used?
Re: Mobile phase of HPLC
Posted: Thu Apr 28, 2011 7:26 am
by DSP007
What a "nitrates and nitrites" - organic (class- alifatic, aromatic , geterocicles, others, any functional grops - amines, acids, phenols,amides , other) or inorganic?
If inorganic -"shotguns do not clean brick (с)" . Need use anionic sorbent.
Re: Mobile phase of HPLC
Posted: Thu Apr 28, 2011 10:08 am
by weitze
I use sodium nitrate which is inorganic compound. Can suggest what kind of anionic solvent as my mobile phase?
Re: Mobile phase of HPLC
Posted: Thu Apr 28, 2011 11:51 am
by H.Thomas
Your best option would be an anion exchange column. Look at the sites of Dionex and Metrohm for these.