Chromatography Forum

Hello everyone,

I am a new user of SPME fibre - analysing tomato volatiles. I bought 3 SPME fibre from Supelco last week. Last night, after 6-7 injection, I forgot to retract SPME fibre from injection port – it was sitting on the injection port overnight (am doing manual injection). When I came back today morning, I noticed fibre inside got burned out and blackened. What could be the reason of that? I am using oven temperature 260°C as final temperature of my sample run.
Do you think I can use that fibre or I need to throw that out?

best regards
Pal

Hi Paul,

What is the phase of this fiber? It is known that polyacrylate fibers get brownish after use. Do you have a QC check for your method? If you have, you can run your QC and compare it with previous results to see whether the fiber performance has changed or not. I would say you lost your fiber in the case it is too much burnt, but a QC check can confirm you this.

Good luck!!!

Erica

Note that the fiber was at the inlet temperature. Check the literature on the fiber and see what it has for temperature limits - that may help.

What was the inlet temperature ?, the final oven temperature is not the temperature that the fiber was exposed to.

Peter

in first place you should 've have seen the manufacturer ' recommendation on how to activate the fibre (am sure with seplco there is always a recommendation ) so check them .and try the QC approach as well but I think from my experience that u lost ur fibre