Chromatography Forum

My lab technician told me a problem.

It is regarding this Agilent 8453 with dissolution tester system, we have problem with missing spectrum problem.

Actually the missing spectrum does not always miss the same region. it may be 400 ~ 500 nm this time & 200~300 next time.

The method that we use in under "multibath dissolution testing" instead of our " standard" mode. The interesting is that when we use the "standard mode" to do the scanning, i got the full spectrum but once i change to the "multibath dissolution testing", the error may occur ( frequently )

The error message that pop out during this missing spectrum is "Invalid data points in spectrum" . Once we press F1 for help, it gives troubleshooting guide on : purge bubbles ; clean the cell, choose a blank , replace SDA board ; replace SPM board . For your info , i use air & filter for the scanning, so the sample does not interfere the result.

Do you think that this is a software problem instead of hardware ?

i need to ask on the CAG Bootp server.

Every time i start the CAG Bootp Server, i must agree on the terms & condition ( like first time software installation ) . And for the CAG Booto Server window, there will be one line stated error : "hardware address not found in BOOTPTAB : ..."
However, i can link with all the peristatic pump, 8 port valve & the 8 multicell

What can be wrong? Advice is much appreciated.

Mitch

Good afternoon.

Of the spectrum, I do not understand. You get a range of calibration solution to check the device, but can not get the range in samples dissolyut test?
This suggests that the spectrophotometer itself is not defective.
Possible reasons for this.
1) Clogged suction filters in vials dissolving excipients (lactose).
The problem is solved by replacing the filter
2) Failure pumping peristaltic pump.
Need to see whether he turns and goes through him, whether the test solution.
3) Clogged valve switch.
4) The program control computer failure or a conflict of devices if they are connected to one computer.
5) Other.

If you urgently as "a knife to her throat" - "if will clogged , and damage gun automatic - must pull the bolt manually"