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HMW calibrant for MALDI-TOF

Posted: Tue Apr 19, 2011 11:29 am
by Mattias
Hi,

I know this is not chromatography, but anyhow:

Does anyone know of a compound that can be used for mass calibration of MALDI-TOF in the mass range of 250-350 kDa?

happy Easter
/Mattias

Re: HMW calibrant for MALDI-TOF

Posted: Tue Apr 19, 2011 6:17 pm
by bhuvfe
I don't use MALDI but maybe you should be able to calibrate it with polymers standards. http://www.polymer.de/products/polymer- ... mbers.html

A high molecular weight protein that creates also clusters (dimer, trimers, etc) might do the trick.