docetaxel extraction issues
Posted: Wed Apr 13, 2011 2:57 pm
Hello. I am having difficulty extracting docetaxel from mouse tissue that has been treated with docetaxel. I have read several protocols using acetonitrile solvent to homogenize the organ and then using the supernatant (solvent) in the subsequent steps for analysis. However, my samples of homogenized tissue in acetonitrile do not demonstrate the necessary docetaxel peak.
I have conducted control studies with just docetaxel and paclitaxel alone and I have seen the peaks (indicating it is not an instrument issue). I have also taken blank tissue (not treated with docetaxel drug), homogenized it in acetonitrile, used paclitaxel for internal control and upon HPLC analysis saw a weaker paclitaxel peak than compared to free paclitaxel (as mentioned above). This leads me to believe that the acetonitrile solvent is not extracting the drug from the tissue.
Any suggestions please???
I have conducted control studies with just docetaxel and paclitaxel alone and I have seen the peaks (indicating it is not an instrument issue). I have also taken blank tissue (not treated with docetaxel drug), homogenized it in acetonitrile, used paclitaxel for internal control and upon HPLC analysis saw a weaker paclitaxel peak than compared to free paclitaxel (as mentioned above). This leads me to believe that the acetonitrile solvent is not extracting the drug from the tissue.
Any suggestions please???