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- Posts: 10
- Joined: Sat May 07, 2011 5:50 pm
hello sir,
we have developed a method for the drug,the method seems to be good while analysing the samples continously ,the peak shapes are good and everything is ok,but we face the problem when we repeat the same analysis after washing the column with water.
The problem is the peaks are going wrong shapes that is they are getting splited and tailing peaks, we face the same problem with two columns.the columns are waters symmetry and x-terra
we are using potassium di hydrogen phosphate buffer with ph 7.8,the ph has been adjusted with 2% KOH solution.
can any one plz explin what the problem
regards malleswar
we have developed a method for the drug,the method seems to be good while analysing the samples continously ,the peak shapes are good and everything is ok,but we face the problem when we repeat the same analysis after washing the column with water.
The problem is the peaks are going wrong shapes that is they are getting splited and tailing peaks, we face the same problem with two columns.the columns are waters symmetry and x-terra
we are using potassium di hydrogen phosphate buffer with ph 7.8,the ph has been adjusted with 2% KOH solution.
can any one plz explin what the problem
regards malleswar
