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Column Bleeding

Discussions about GC-MS, LC-MS, LC-FTIR, and other "coupled" analytical techniques.

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Column Bleeding

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Davidantony
Dear All,
In one our C-18 column(Agilent zorabax SB C-18 2.1x150mm,5µ)it is found that the column particles are coming outside.The maximum flow is 0.4ml/min.Why this type of problem coming how to stop this type of column bleeding

David Antony

Re: Column Bleeding

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Bigbear
I hate to ask, but have you installed it correctly? Some manufacturers put a frit with larger pore size in the inlet . If that is your case some packing could leak from the column.
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