peak fronting at higher temperature

[MestizoJoe]

I was wondering if anyone could speculate on why I observe fronting at higher temperatures for one peak.

The peak is likely a cation, a related substance of a ''''pril type drug. It also has a carboxyl group. The column is a phenomenex polar rp (phenyl) column using 99% [0.1%TFA aq] 1% MeOH.


this is at 35C


at 50C

[zokitano]

Are you sure that your compound is stable at 50C? The cause for such "fronting" could be in some cases due to the presence of "new" compounds generated by temperature mediated conversion/degradation of some of the initial analytes in the sample.