Reference Bandwidth for DAD

[Mike H.]

I've read the help files for reference bandwidth for the DAD. Supposedly using one minimizes refractive index changes in your baseline, but I've never really seen a difference in the chromatography whether you use a reference bandwidth or not. Does it really do anything? If it does what guidelines should I follow when choosing one? thx

[zokitano]

If you noticed for your particular separation that using the reference wavelength or not doesn't make difference in your final chromatograms, always good advice is: do NOT use it.

Regards