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- Posts: 33
- Joined: Mon May 25, 2009 5:53 am
Hi guys,
in the past when I acquired data, the EM voltage would run consistently at the same voltage for each injection, which would always be the autotune voltage +12 relative.
Recently however, I have noticed fluctuations in sensitivity and when I checked the saved method parameters for each data file, I noticed that the EM voltage was different for *each* injection (eg. 2247, 2011, 2294). I thought the whole point of the autotune was to get the best operating voltage which is then saved and used for all the subsequent analyses.
Why would the voltages change? Could my atune file be corrupted?
cheers
Liz
in the past when I acquired data, the EM voltage would run consistently at the same voltage for each injection, which would always be the autotune voltage +12 relative.
Recently however, I have noticed fluctuations in sensitivity and when I checked the saved method parameters for each data file, I noticed that the EM voltage was different for *each* injection (eg. 2247, 2011, 2294). I thought the whole point of the autotune was to get the best operating voltage which is then saved and used for all the subsequent analyses.
Why would the voltages change? Could my atune file be corrupted?
cheers
Liz
