Surfactin doubts

[Gilberto]

Good morning people;

I have some doubts about separation.

I need to quantify a product called surfactin, but the standard is a surfactin mix. It has many pikes. But I have problem with 2 pikes.

see this...



What I have to do?

My mobil phase (isocratic, 1,4 mL/min) ACN:Water:TFA (80:20:10)

Thanks people

[tom jupille]

What is the problem (difficult to tell anything from the small image).