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precaution for usgae at pH 9??

http://www.chromforum.org/viewtopic.php?t=16155

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precaution for usgae at pH 9??

Posted: Fri Mar 25, 2011 4:18 am
by rick1112
Hi

I would like to know what all precautions (or changes to system) should I take of using regular Agilent 1200 series HPLC with mobile phase pH of 9 ??

Thanks

Re: precaution for usgae at pH 9??

Posted: Fri Mar 25, 2011 8:33 am
by danko
Non

Best Regards

Re: precaution for usgae at pH 9??

Posted: Mon Mar 28, 2011 5:10 am
by rick1112
will there be no effect on the tubings and pump??

Re: precaution for usgae at pH 9??

Posted: Mon Mar 28, 2011 5:49 am
by bisnettrj2
Per a document on Agilent's website:

"In general, 1200 modules operate over a pH range 1 to 12.5.

pH > 9.5
• Replace standard (Vespel) rotor seals in all rotary valves with either
Tefzel or PEEK seals.
• Replace the standard glass solvent inlet filters with stainless steel inlet
filters.
• Be aware that quartz flow cell windows are slowly etched. Don’t let
high pH solvents stand in the flow cell for long periods."

http://www.chem.agilent.com/Library/sli ... 8_Tour.pdf

So, you're at pH 9, which is right on the border of their precautions, so it might be a good idea to swap the recommended parts just to be safe.
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