Determining the isocratic void using a short column

[IsotopeC12]

I have a problem compound that I can only get good chromatography for using this scenario:

20/80 H2O/ACN

Thermo Scientific Hypersil Gold, 2.1 x 50mm, 1.9µ

0.600mL/min flow rate

Retention time is 0.17 minutes

Total Run time is 3.0 minutes

I know this is very early, and I use gradient methods 99% of the time, but the peak shape is nice and the experiment passes. What would be an acceptable retention time in this case? Is any retention time acceptable when using isocratic?

[carls]

RT 0.17min is at the void time of the column. What does the chromatoghraphy look like (tailing, fronting?) at lower %ACN? In the case of an unretained compound you might as well just dissolve your sample and measure the absorbance using a UV-vis spectrophotometer.