precaution for usgae at pH 9??

[rick1112]

Hi

I would like to know what all precautions (or changes to system) should I take of using regular Agilent 1200 series HPLC with mobile phase pH of 9 ??

Thanks

[danko]

Non

Best Regards

[rick1112]

will there be no effect on the tubings and pump??

[bisnettrj2]

Per a document on Agilent's website:

"In general, 1200 modules operate over a pH range 1 to 12.5.

pH > 9.5
• Replace standard (Vespel) rotor seals in all rotary valves with either
Tefzel or PEEK seals.
• Replace the standard glass solvent inlet filters with stainless steel inlet
filters.
• Be aware that quartz flow cell windows are slowly etched. Don’t let
high pH solvents stand in the flow cell for long periods."

http://www.chem.agilent.com/Library/sli ... 8_Tour.pdf

So, you're at pH 9, which is right on the border of their precautions, so it might be a good idea to swap the recommended parts just to be safe.