acetaminophen analysis with FID

[maria scjut]

Hello,

I'm trying to quantify acetaminophen by GC FID but I would like to know if I can do it without IS? What do you recommend me to use instead of IS? (at this moment I can't afford one). Regarding derivatization, is it necessary in this case? I was thinking to use LLE without derivatization. I'm novice in chromatography and I'm sorry for that.

Thank you

[Consumer Products Guy]

Acetaminophen is readily detected by HPLC using UV. Likely that your lab doesn't have that, though. I'm not a huge fan of "making do" when HPLC is such a standard piece of equipment these days. And, if this is for pharmaceutical analysis (which is likely), HPLC would be the best to use.

That stated: for any GC I would make trimethylsilyl derivatives, and use external standard quantitation. I'm assuming that you have a GC autosampler; if not, your lab is 1970s.

[maria scjut]

Acetaminophen is readily detected by HPLC using UV. Likely that your lab doesn't have that, though. I'm not a huge fan of "making do" when HPLC is such a standard piece of equipment these days. And, if this is for pharmaceutical analysis (which is likely), HPLC would be the best to use.

That stated: for any GC I would make trimethylsilyl derivatives, and use external standard quantitation. I'm assuming that you have a GC autosampler; if not, your lab is 1970s.

my lab is not equiped with HPLC yet, but I hope that in future (aprox. 2 month) it will be purchase. I do have a GC autosampler and i'm waiting to receive an MS for my GC next month (I'm hoping that it will be much easier then), anyway until that day I must quantify acetaminophen tomorrow and I'm very cloudy at this moment about it, but thank you

[JGK]

As said previously HPLC is the way to go. However I did find this reference from 1976 http://www.clinchem.org/cgi/reprint/22/6/879.pdf

[maria scjut]

As said previously HPLC is the way to go. However I did find this reference from 1976 http://www.clinchem.org/cgi/reprint/22/6/879.pdf

Thank you very much,

I'll try on GC FID because in this moment is the only possible way to do it.

Best wishes,