urease inhibitor (NBPT) in soil or in cow slurry
Posted: Fri Mar 11, 2011 10:36 am
Dear all,
I am looking for any advice that could help me set up a HPLC methodology that allows the measurement of urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT, also called nBTPT) concentration in soil or in cow slurry. I intend to monitor NBPT level over time after addition to incubated soil or slurry samples. So far, I have found very little in the literature apart from Douglass and Hendrickson (1991) paper. I haven't tested that yet (I don't have an easy access to a HPLC myself), but I was wondering if anyone knew about any other HPLC methodology (if there is any).
Regards,
eddy
Douglass, E.A. and Hendrickson, L.L. (1991) HPLC method for the analysis of urea inhibitor N-(n-butyl) thiophosphoric triamide and its metabolites. Journal of Agricultural and Food Chemistry, 39 (12): 2318-2321.
I am looking for any advice that could help me set up a HPLC methodology that allows the measurement of urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT, also called nBTPT) concentration in soil or in cow slurry. I intend to monitor NBPT level over time after addition to incubated soil or slurry samples. So far, I have found very little in the literature apart from Douglass and Hendrickson (1991) paper. I haven't tested that yet (I don't have an easy access to a HPLC myself), but I was wondering if anyone knew about any other HPLC methodology (if there is any).
Regards,
eddy
Douglass, E.A. and Hendrickson, L.L. (1991) HPLC method for the analysis of urea inhibitor N-(n-butyl) thiophosphoric triamide and its metabolites. Journal of Agricultural and Food Chemistry, 39 (12): 2318-2321.