Chromatography Forum

Hi!

I need to do some SPE to separate free fatty acids, TRG's, squalene, and cholesterol esters from a lipid extract of sebum. The isolated samples will then be analyzed by GC/MS. I'm not concerned with keeping the TRG's intact, so I thhink GC/MS should be fine.

I apprciate any with this.
Thanks!

Here's a paper worth looking up.


A procedure for fractionation of sphingolipid classes by
solid-phase extraction on aminopropyl cartridges
J. Bodennec,1,*,§ O. Koul,† I. Aguado,* G. Brichon,* G. Zwingelstein,* and J. Portoukalian§
Institute Michel Pacha,* University Claude Bernard-Lyon I, 83500 La Seyne sur Mer, France; Eunice Kennedy
Shriver Center for Mental Retardation,† Waltham, MA 02452 and Department of Neurology, Harvard Medical
School and Massachusetts General Hospital, Boston, MA 02214; and Laboratory of Tumor Glycobiology,§
Faculty of Medicine Lyon-Sud, University Claude Bernard Lyon-I, 69921 Oullins, France