HPTLC Method Validation

[viru]

is tthere any difference between parameters of mehtod validation for HPLC and HPTLC. when the LOD and LOQ require to determine.

[tom jupille]

In my view, yes, with the hopefully obvious proviso about analogous parameters (Rf instead of k', etc.).

[aniket]

tahnk you for replying
what should be the acceptance critrion for the SD for limearity curve in HPTLC method validation ?
as in case of HPLc method validation it should not be > 2,

and i am getting SD 4.12 for linearity curve in HPTLC method validation?

is at acceptable?

Pls help me on this

[tom jupille]

If you are using a densitometer to quantitate, you should use an appropriate fitting function for calibration (not necessarily linear). Alternatively, use a narrower range (any detector is approximately linear over a sufficiently narrow range).