Chromatography Forum

There are some scientific things such as papers , books , presentations or seminars etc that someone can forget them simply but there are some of these cann't be forgotten

Please participate in this informal post to learn from you the distiction things ......

This is the most memorable paper i have come a cross that was helpfull for me
(( Before the injection—modern methods of sample preparation for separation techniques )) , It is the best from 2002 -2007

Ref : http://www.lboro.ac.uk/departments/cm/i ... b%2008.GIF

Yes, there are obviously ground-breaking papers, and there are little ones that are life-changing! As well as beautiful ones that are just lovely to read.

Here's an odd one in the "little but life-changing" category; it's such a useful tool. It's how to make buffers with multiple components, able to buffer over a wide pH range without varying ionic strength. And absolute must if you do enzyme pH-dependence studies.
Ellis KJ, Morrison JF (1982) Buffers of constant ionic strength for studying pH-dependent processes. Methods Enzymol 87: 405-42

For sheer beauty, and cutting-edge, here's one in which someone has labelled biological material with 13C to the point that everything is 100% 13C, thereby creating the ideal internal standard for non-labelled studies, and also finding the amount of carbon in every single unidentified peak in one go:
13C Isotope-Labeled Metabolomes Allowing for Improved Compound Annotation and Relative Quantification in Liquid Chromatography-Mass Spectrometry-based Metabolomic Research. Giavalisco et al., Analytical chemistry 81:6546-6551

Other papers that have been memorable for me have been the works of Kacser and Burns on control coefficients, and a load of stuff on bacterial cell walls based on soap bubbles, which appealed when I was a student, but I gather it's all fallen out of favour since.

Being a HILIC person my favourite is of course
A.J. Alpert, J. Chromatogr. 499 (1990) 177-196

An other favourite that is very funny and ground breaking is.
(the paper ending the discussion on the retention mechanism in normal phase chromatography)
Snyder, L. R., Poppe, H., J. Chromatogr. 1980, 184, 363–413.