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immobilized stationary phase

http://www.chromforum.org/viewtopic.php?t=15779

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immobilized stationary phase

Posted: Mon Feb 14, 2011 12:03 pm
by vvreddy
Is any one know what is immobilized stationary phase in HPLC chiral column.
what is difference between coated and immobilized stationary phase.
In Chiralpak AD packing composition is
amylose tris (3,5-dimethyl phenyl carbamate) coated on silica .
Where as in Chiralpak-IA it is
amylose tris (3,5-dimethyl phenyl carbamate) immobilized on silica.
what is the diffrence?

Re: immobilized stationary phase

Posted: Mon Feb 14, 2011 2:34 pm
by tom jupille
"Coated" is just what it sounds like; the layer of stationary phase is like a coat of paint on the support. It is not chemically attached and so is somewhat fragile.

The immobilized phase is chemically bound to the substrate.

Re: immobilized stationary phase

Posted: Tue Feb 15, 2011 4:10 am
by vvreddy
Thank you very much sir.
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