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HPTLC Method Validation
Posted: Wed Mar 02, 2005 11:09 am
by viru
is tthere any difference between parameters of mehtod validation for HPLC and HPTLC. when the LOD and LOQ require to determine.
Posted: Wed Mar 02, 2005 2:45 pm
by tom jupille
In my view, yes, with the hopefully obvious proviso about analogous parameters (Rf instead of k', etc.).
thanks
Posted: Fri Mar 04, 2005 5:24 am
by aniket
tahnk you for replying
what should be the acceptance critrion for the SD for limearity curve in HPTLC method validation ?
as in case of HPLc method validation it should not be > 2,
and i am getting SD 4.12 for linearity curve in HPTLC method validation?
is at acceptable?
Pls help me on this
Posted: Fri Mar 04, 2005 12:47 pm
by tom jupille
If you are using a densitometer to quantitate, you should use an appropriate fitting function for calibration (not necessarily linear). Alternatively, use a narrower range (any detector is approximately linear over a sufficiently narrow range).