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how can i retened polar compounds in RPLC like hcl salts

Posted: Fri Feb 04, 2011 5:30 pm
by tirajesh
here i am facing problem with temozolamide Impurities one of the impurity is very polar (dacarbazole impurity) it is in usp, we are following usp method but is not retaining it RT is 2.0 pls give solution for this

Re: how can i retened polar compounds in RPLC like hcl salts

Posted: Sun Feb 06, 2011 3:14 pm
by carls
please post the separation conditions you are using