-
- Posts: 656
- Joined: Tue Jul 05, 2005 7:45 am
It is quite easy to understand why there can be peaks from previous injections in isocratic mode (the peak continously move through the column), but how does it work in gradient mode?
I have a method that contains a gradient from 3 - 30% acetonitrile. When I inject a sample, the end of the gradient looks nice and clean. But when I inject the next sample or blank, I get a couple of nice sharp peaks in the end of the gradient that must come from the previous sample. I don't think I have ever seen this in gradient mode? Any thoughts?