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HPLC Column selection for an acid sensitive analyte

http://www.chromforum.org/viewtopic.php?t=1528

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HPLC Column selection for an acid sensitive analyte

Posted: Thu Feb 24, 2005 4:37 am
by jeegar
I am looking to buy a C18 column, 15 cm, 5 um dp and 4-4.6 id, for my reversed phase HPLC application. The analyte is acid sensitive, therefore, I should work with a mobile phase with a pH of about 8. I was wondering what column I should use to tolerate the alkaline pH range and yet not expensive.

I would certainly appreciate your help.

Sincerely,
Jeegar

Posted: Fri Feb 25, 2005 1:06 am
by tom jupille
Many manufacturers now have columns stable at pH 8. This is not an inclusive list, but:

Waters Xterra
Phenomenex Luna
Agilent Eclipse

come to mind

Posted: Fri Feb 25, 2005 2:20 am
by Kostas Petritis
Agilent Extend and Phenomenex Gemini to name some more...

Posted: Fri Feb 25, 2005 5:38 am
by jeegar
Dear Tom and Kostas,

Thank you for your response. I will look into them.

Jeegar
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