Problem with HPLC - one line has no pressure

[dkreller]

Hi,

After changing one of the reservoirs for my Alliance 2695, I discovered that the system won't prime (either wet or dry) on the line for which I just changed the bottle. Talked to tech support who said that if there's no pressure on any of the lines, it's a check-valve problem. But however, the system will prime on all other lines. I am kind of new to troubleshooting, but imagine that a clear place to start would be to check a filter or input line.

Any advice? Thanks for your time,

Dave

[JA]

for the mobile phase filter, simply try to prime without it.

this might be too far ahead, but we once had a blown membrane in the gradient proportioning valve, GPV. If you have a suitable service contract just call the engineers out to look at it, if not, bear this component in mind.