Chromatography Forum

I will have 1 uM magnesium sulphate in my sample and I am using C8,3.5 micron column with water:acn gradient. I am worried it cause problem to my column and ion suppression in my lcmsms..please help

thanks

First of all, 1 micromolar is a *very* low concentration (130 micrograms/L = 130 nanograms/mL = 130 picograms/microliter), so you probably aren't injecting more than a few nanograms. Second, MgSO4 should be essentially unretained under reversed-phase conditions, so it will elute at t0. I wouldn't worry.

thanks tom