Advertisement

magnisum sulphate in my sample

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

3 posts Page 1 of 1
I will have 1 uM magnesium sulphate in my sample and I am using C8,3.5 micron column with water:acn gradient. I am worried it cause problem to my column and ion suppression in my lcmsms..please help

thanks
First of all, 1 micromolar is a *very* low concentration (130 micrograms/L = 130 nanograms/mL = 130 picograms/microliter), so you probably aren't injecting more than a few nanograms. Second, MgSO4 should be essentially unretained under reversed-phase conditions, so it will elute at t0. I wouldn't worry.
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
thanks tom
3 posts Page 1 of 1

Who is online

In total there are 22 users online :: 2 registered, 0 hidden and 20 guests (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am

Users browsing this forum: Ahrefs [Bot], Bing [Bot] and 20 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry