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Sample solvent for method development

http://www.chromforum.org/viewtopic.php?t=15181

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Sample solvent for method development

Posted: Fri Jan 07, 2011 4:26 pm
by annaand
I have a very basic question: While developing an RP-HPLC method by increasing an aqueous phase percent in mobile phase, what solvent should be used to prepare sample? Richer in organic or aqueous phase?

Re: Sample solvent for method development

Posted: Fri Jan 07, 2011 5:21 pm
by tom jupille
In general, the diluent should be the same as the mobile phase. When that is not possible or practical, more aqueous is better.
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