the most important with me

[vinhvinh1979g]

Hi everybody
I am running HPLC to separate a mixed organic acid( citric acid, oxalic acid) with condition: flow rate 0.4ml/min; pH=6; wave length 210nm; acclaim trinity p1( Dionex). I have problem: no peak. i think wave length is wrong. you suggest for me. thank.

[tom jupille]

Possibilities:
1. You have not injected a high enough concentration
4. Your detector sensitivity is not set high enough
2. Your mobile phase is too weak and your compounds never elute
3. Your mobile phase is too strong and all your compounds elute at t0 (do you see anything at all?)
5. Your detector is malfunctioning

So: a bit more information would be useful:
what are the concentrations of your analyte?
- what injection volume?
- what sensitivity on the detector?
- what is your mobile phase?
- are you using a variable wavelength detector or a diode array?
- have you checked the wavelength calibration on the detector?
- are you trying to duplicate an existing method or are you developing the separation "from scratch"?