Chromatography Forum

Hoping someone can help with some advice. I haven't used an HPLC is quite a long time (last time I did it was running on an old VAX computer), and I recently purchased a Waters E2695 Alliance model. I've been running through old sample prep procedures in my head, and I'm trying to acquire the needed items for my lab. My old company had a house vacuum line I could plug into for filtering and degassing my mobile phases. I don't have a vacuum line here, so I need to purchase a small vacuum pump for this purpose.I don't have a ton of money to spend, and have found some online in the $300-500 range. I can do that without much issue. Any advice on a low cost purchase would be greatly appreciated. Thanks.

https://ecat.fishersci.ca/(S(xfyq4qzvjo ... cid=158910 We used to use one of these for filtration

If you don't mind putting a small amount of solvent down the drain, you can get a water aspirator for <20$.

Sonication is often a suitable means of degassing for HPLC. Also note that modern HPLC's are less in need of degassed solvents than were those of the 1980's. However, degassing is never a bad idea -- unless you continue it so long as to change the nature of a solvent mixture.

if you have an E2695 or a 2695 then these should have a vacuum degasser installed. i think it was the 2690 that had helium sparge, or vacuum degasser as an option. to check, on the main screen / or next page depending on how you have the screens set up you should find degasser, highlight this and then using the cursor key select on. other thing to check is open both of the doors at the bottom, on the right side you should see the gradient valve with 5 lines coming out of it, 1 ( centre ) going to the first pump head, the other 4 going to vacuum chambers which have corresponding lines coming out of them to your solvent bottles

For filtering, you'll probably get away with a simple water-tap-pump. I'll admit my lab no longer filters organic solvents (except after adding solids) as we came to the conclusion that the big chemical suppliers are better at filtering than us.

Hopefully. by now you have found out that the Waters 2695 comes equipped with an internal membrane type degasser.
Call me if you have questions. We do a lot of work on these Alliance systems.
Tony Vella www.hplcworks.net 8586637051

Hoping someone can help with some advice. I haven't used an HPLC is quite a long time (last time I did it was running on an old VAX computer), and I recently purchased a Waters E2695 Alliance model. I've been running through old sample prep procedures in my head, and I'm trying to acquire the needed items for my lab. My old company had a house vacuum line I could plug into for filtering and degassing my mobile phases. I don't have a vacuum line here, so I need to purchase a small vacuum pump for this purpose.I don't have a ton of money to spend, and have found some online in the $300-500 range. I can do that without much issue. Any advice on a low cost purchase would be greatly appreciated. Thanks.