Sebum analysis by HPLC [June 15, 2004]
Posted: Tue Aug 24, 2004 9:40 pm
By spolygon on Tuesday, June 15, 2004 - 02:32 am:
Hi!
I am a beginner. I am trying to analyze the various components of sebum by HPLC. I am looking at help on what column/mobile phase and methods would be best.
Thanks
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By Anonymous on Friday, June 18, 2004 - 07:52 pm:
what is sebum?
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By spolygon on Wednesday, June 23, 2004 - 01:23 am:
Hi!
Sorry for the delay in responding. Sebum is the stuff that is secreted by sebaceous glands in the skin and is responsible for the oily feeling on the skin. It is a mixture of fatty acids, triglycerides,
cholestrol, squalene, wax esters etc.
I am looking for a method that can distinguish between the classes of compounds mnentioned above. If individual fatty acids etc. can be resolved it would be nice but is not absolutely necessary. Thanks again for your help.
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By HW Mueller on Wednesday, June 23, 2004 - 03:04 am:
Try TLC first (check lipids in any book on TLC).
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By Anonymous on Wednesday, June 23, 2004 - 08:41 am:
It can be done with HPLC, but the problem will be a suitable detection technique. What is your detector?
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By Anonymous on Friday, June 25, 2004 - 02:08 am:
what about using a GC method?
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By Anonymous on Thursday, July 1, 2004 - 02:17 am:
thanks for the responses. I have access to a UV and RI detector. In addition a light scattering and FID detrector could be arranged if necessary.
Which dtector system do you advise?
I am trying to study oxidation products of sebum. The stuff that causes yellowing of soiled garments over time. The high molecular weight compunds produced are difficult to analyze by GC. Polar molecules also pose a problem with GC. I was hoping to use HPLC to complement my GC analysis. Fill in the 'holes' so to speak.
Thanks again for your help.
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By HW Mueller on Friday, July 2, 2004 - 12:01 am:
Looks like you found your life´s job. If those substances are yellow and can be dissolved then a UV/VIS detector will do. How do you get this stuff off the cloth?
Still, seems like a typical exploratory job for TLC.
Hi!
I am a beginner. I am trying to analyze the various components of sebum by HPLC. I am looking at help on what column/mobile phase and methods would be best.
Thanks
-------------------------------------------------------------------------------------------------------
By Anonymous on Friday, June 18, 2004 - 07:52 pm:
what is sebum?
-------------------------------------------------------------------------------------------------------
By spolygon on Wednesday, June 23, 2004 - 01:23 am:
Hi!
Sorry for the delay in responding. Sebum is the stuff that is secreted by sebaceous glands in the skin and is responsible for the oily feeling on the skin. It is a mixture of fatty acids, triglycerides,
cholestrol, squalene, wax esters etc.
I am looking for a method that can distinguish between the classes of compounds mnentioned above. If individual fatty acids etc. can be resolved it would be nice but is not absolutely necessary. Thanks again for your help.
-------------------------------------------------------------------------------------------------------
By HW Mueller on Wednesday, June 23, 2004 - 03:04 am:
Try TLC first (check lipids in any book on TLC).
-------------------------------------------------------------------------------------------------------
By Anonymous on Wednesday, June 23, 2004 - 08:41 am:
It can be done with HPLC, but the problem will be a suitable detection technique. What is your detector?
-------------------------------------------------------------------------------------------------------
By Anonymous on Friday, June 25, 2004 - 02:08 am:
what about using a GC method?
-------------------------------------------------------------------------------------------------------
By Anonymous on Thursday, July 1, 2004 - 02:17 am:
thanks for the responses. I have access to a UV and RI detector. In addition a light scattering and FID detrector could be arranged if necessary.
Which dtector system do you advise?
I am trying to study oxidation products of sebum. The stuff that causes yellowing of soiled garments over time. The high molecular weight compunds produced are difficult to analyze by GC. Polar molecules also pose a problem with GC. I was hoping to use HPLC to complement my GC analysis. Fill in the 'holes' so to speak.
Thanks again for your help.
-------------------------------------------------------------------------------------------------------
By HW Mueller on Friday, July 2, 2004 - 12:01 am:
Looks like you found your life´s job. If those substances are yellow and can be dissolved then a UV/VIS detector will do. How do you get this stuff off the cloth?
Still, seems like a typical exploratory job for TLC.