Chromatography Forum

I plan to analyze a sugar mixture glucose, fructose and sucrose in sugar cane
via HPLC.
CAn anyone coment what are the advateges and disadvantages of this two column types?
Are all the sugars quantificable with both of them?
Any other comment?

I have used a carbohydrate column with refractive index detector before and the results were very good.

What do you mean by carbohydrate column? If you mean what Agilent specify as their "carbohydrate column" then this is just a stabilised amino column anyhow.

We did develop a method for the sugars you've listed using that column and it was pretty reliable. However, the only thing I would say is that the LOQ/LOD isn't particularly great mainly due to the use of an AcN/water mobile phase giving quite a high background noise with refactive index detection (RID). If you want lower levels of detection then I would suggest you use one of the proprietary cationic resin columns that have also been used for sugar analysis. Thay use just pure water as the mobile phase and offer significantly lower noise levels when using RID.

ccharles

The two columns in question are either :

1) a silica matrix with and amino modification
2) a sulphonated polystyrene/ DVB resin

both can be used for the analysis of carborhydrates.

The question on which is best is mixed and can only be answered by the user?

Is this for research or production?
Is cost of the mobile phase important?
What's the limits of acceptable results?
What other compounds are in the mix?

The seperation is not difficult the best way to do is.

Enclosed is a web page from Macherey-Nagel that shows this seperation using water at the mobile phase.

https://www.mn-net.com/web/MN-WEB-appli ... WIK-4XQFUR

We have other chromatograms showing many different columns.

www.mn-net.com