Chromatography Forum

Hello,

usually I use original Gerstel 10 µL syringes, but this order I received Hamilton bevel type syringe for a replacement. And the needle could be replaced, as far as I understand. (Code: G6500-80115.) Looks like it will fit Gerstel autosampler, but I am not sure if bevel type needle is good choise for GC. Any advices are welcomed and appreciated.

P.S. Didn't dig too far and could only find one post suggesting to void bevel type syringes for GC.

You can use them. There is some argument about how quickly you will core a septum and risk of plugging the needle. I'll have to look, but I believe my instrument is curently using bevel type syringes. Septum and liner are changed daily, so it is unlikely there will be a problem with coring a septum.

I have to disagree with Don on this - beveled needle tips are a disaster - even if they do not actually core a chunk out of the septum they have a tendency not to follow the same hole every time, and the multiple holes lead more quickly to leaks. If you are rpelacing septa daily for other reasons then this might not ne an issue.

Peter

I lean toward Peter's opinion rather than Don's. However, if properly aligned and beveled, a needle should not wear out a septum for many, perhaps scores of injections before ruining a septum.

Side opening needles are useful but injecting sideways into an injection port doesn't always bode well.

Probably the best choice is the tapered tip needle, introduced by Agilent if I remember correctly, when used with a quality septum if used with an autosampler.

Opinions will vary on this and it is probably dependent upon your application and whether one is performing automated or manual injections.

I used to perform manual injections with a beveled needle syringe and replaced my septum, whether I needed to or not, when the instruments were cold on Monday mornings.

best wishes,

Rod

Thank you for replies.

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